Lipidomic analysis of plasma lipoprotein fractions in myocardial infarction-prone rabbits

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• 作者：Hiroaki Takeda¹ ; ⁴ ; Tomonari Koike² ; ³ ; Yoshihiro Izumi¹ ; ⁴ ; Takayuki Yamada⁴ ; Masaru Yoshida⁵ ; ⁶ ; Masashi Shiomi² ; ³ ; Eiichiro Fukusaki⁴ ; Takeshi Bamba¹ ; ⁴ ; ^{bamba@bioreg.kyushu-u.ac.jp" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Lipidomics ; Lipoprotein ; Supercritical fluid chromatography mass spectrometry ; Coronary artery disease ; Myocardial infarction-prone Watanabe heritable hyperlipidemic rabbit ; Phosphatidylethanolamine plasmalogen
• 刊名：Journal of Bioscience and Bioengineering
• 出版年：2015
• 出版时间：October 2015
• 年：2015
• 卷：120
• 期：4
• 页码：476-482
• 全文大小：1153 K

文摘

Lipids play important roles in the body and are transported to various tissues via lipoproteins. It is commonly assumed that alteration of lipid levels in lipoproteins leads to dyslipidemia and serious diseases such as coronary artery disease (CAD). However, lipid compositions in each lipoprotein fraction induced by lipoprotein metabolism are poorly understood. Lipidomics, which involves the comprehensive and quantitative analysis of lipids, is expected to provide valuable information regarding the pathogenic mechanism of CAD. Here, we performed a lipidomic analysis of plasma and its lipoprotein fractions in myocardial infarction-prone Watanabe heritable hyperlipidemic (WHHLMI) rabbits. In total, 172 lipids in plasma obtained from normal and WHHLMI rabbits were quantified with high throughput and accuracy using supercritical fluid chromatography hybrid quadrupole-Orbitrap mass spectrometry (SFC/Q-Orbitrap-MS). Plasma levels of each lipid class (i.e., phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, lysophosphatidylcholine, lysophosphatidylethanolamine, sphingomyelin, ceramide, triacylglycerol, diacylglycerol, and cholesterol ester, except for free fatty acids) in 21-month-old WHHLMI rabbits were significantly higher than those in normal rabbits. High levels of functional lipids, such as alkyl-phosphatidylcholines, phospholipids including 蠅-6 fatty acids, and plasmalogens, were also observed in WHHLMI rabbit plasma. In addition, high-resolution lipidomic analysis using very low density lipoprotein (VLDL) and low density lipoprotein (LDL) provided information on the specific molecular species of lipids in each lipoprotein fraction. In particular, higher levels of phosphatidylethanolamine plasmalogens were detected in LDL than in VLDL. Our lipidomics approach for plasma lipoprotein fractions will be useful for in-depth studies on the pathogenesis of CAD.