A deep coverage Dictyostelium discoideum genomic DNA library replicates stably in Escherichia coli
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- 作者：Rafael D. Rosengarten ; Pamela R. Beltran ; Gad Shaulsky ; gadi@bcm.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：Dictyostelium genomic library ; Linear bacteriophage plasmid
- 刊名：Genomics
- 出版年：2015
- 出版时间：October 2015
- 年：2015
- 卷：106
- 期：4
- 页码：249-255
- 全文大小：610 K
文摘
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The genome of Dictyostelium discoideum is very A:T-rich.
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Previous attempts to generate stable genomic libraries in E. coli have been rather unsuccessful.
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A library of D. discoideum genomic DNA is constructed in the pJAZZ vector.
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The library is diverse, unbiased and stable over many rounds of amplification.