Studies on the extraction of the non-heme iron centre from photosystem II of higher plants

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• 作者：Kurreck ; J. ; Garbers ; A. ; Reifarth ; F. ; Parak ; F. ; Renger ; G.
• 刊名：Journal of Inorganic Biochemistry
• 出版年：1995
• 出版时间：August, 1995
• 年：1995
• 卷：59
• 期：2-3
• 页码：389
• 全文大小：64.4 K

文摘

Flash induced transient changes of the chlorophyll a fluorescence and Mössbauer spectroscopy were used to analyse modifications of the non-heme iron centre induced by treatment of trypsinised PSII membrane fragments with LiClO₄ and o-phenanthroline based on a procedure described in with modifications as in. EPR spectroscopy reveals that the magnetic interaction of the non-heme iron with the semiquinone anion radical disappears in these preparations. Kinetic measurements of the flash induced fluorescence yield in the presence and absence of K₃[Fe(CN)₆] show that in these samples i) the electron transfer between the primary and secondary plastoquinone acceptors (Q_A and Q_B) is suppressed (already as a result of trypsination), and ii) the extent of a fast Q_A⁻ reoxidation by preoxidised non heme-iron (Fe³⁺) is largely eliminated.