Antioxidant and anticancer activity of extract from Betula platyphylla var. japonica

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• 作者：Ju ; Eun Mi ; Lee ; Si Eun ; Hwang ; Hyun Jin ; Kim ; Jeong Hee
• 关键词：Antioxidant ; Anticancer ; Apoptosis ; Betula platyphylla var. japonica
• 刊名：Life Sciences
• 出版年：2004
• 出版时间：January 9, 2004
• 年：2004
• 卷：74
• 期：8
• 页码：1013-1026
• 全文大小：422 K

文摘

The antioxidant and anticancer properties of a medicinal plant, Betula platyphylla var. japonica were investigated. The total methanol extract of B. platyphylla var. japonica had protective effects against hydrogen peroxide (H₂O₂) in the Chinese hamster lung fibroblast (V79-4) cell line and induced apoptotic cell death in human promyelocytic leukemia (HL-60) cells, a cancer cell line. B. platyphylla var. japonica extract significantly increased cell viability against H₂O₂. The extract also showed high 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity (IC₅₀ 2.4 μg/ml) and lipid peroxidation inhibitory activity (IC₅₀ below 4.0 μg/ml). Furthermore, B. platyphylla var. japonica extract reduced the number of V79-4 cells arrested in G₂/M in response to H₂O₂ treatment and increased the activities of several cellular antioxidant enzymes, including superoxide dismutase, catalase and glutathione peroxidase. Treatment with B. platyphylla var. japonica extract induced cytotoxicity and apoptosis in HL-60 cells, as shown by nucleosomal DNA fragmentation, increases in the subdiploid cell population, and fluorescence microscopy. B. platyphylla var. japonica extract gradually increased the expression of pro-apoptotic Bax and led to the activation of caspase-3 and cleavage of PARP. These findings suggest that B. platyphylla var. japonica exhibits potential antioxidant and anticancer properties.