Ionic liquid-based aqueous two-phase extraction within a microchannel system
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- 作者：Uro? Novak ; Andrej Pohar ; Igor Plazl ; Polona ?nidar?i?-Plazl
- 关键词：Aqueous two-phase system ; Microfluidics ; Ionic liquid ; Protein extraction
- 刊名：Separation and Purification Technology
- 出版年：2012
- 出版时间：3 September, 2012
- 年：2012
- 卷：97
- 期：Complete
- 页码：172-178
- 全文大小：578 K
文摘
Ionic liquid-based aqueous two-phase systems (IL-ATPSs) are great candidates for the replacement of volatile organic solvents in liquid-liquid extractions. Besides, microfluidic separation techniques are a promising alternative to conventional systems since they provide continuous operation, better performance and easier capacity increase by a numbering-up approach. Herein, the advantages of IL-ATPSs and microfluidic continuous separation were combined within a microfluidic device with parallel flow allowing for the separation of the two phases at the exit of the microchannel. An aqueous solution of [C4mim] [BF4] and d-fructose was used as an IL-ATPS and compared with a conventional ATPS consisted of polyethylene glycol/salt solution. Based on the evaluated partitioning coefficient of a model substance, namely bovine serum albumin, the ionic liquid concentration and pH value of the IL-ATPS have been selected for the use within a pressure-driven microchannel system with y-shaped inlet and outlet. Furthermore, a three-dimensional model considering convection in the flow direction and diffusion in all spatial directions at steady-state conditions was developed, validated by experimental results and used to assess the feasibility of micro-scale parallel flow extraction in a wide range of flow rates. The chosen IL-ATPS was shown to be much more efficient media for continuous microfluidic extraction as compared to conventional ATPS, primarily due to much lower dynamic viscosity of IL-rich phase related to PEG-rich phase.