Cerium dioxide nanoparticles did not alter the functional and morphologic characteristics of ram sperm during short-term exposure

详细信息    查看全文

• 作者：Laura Falchi^a ; ^{lfalchi@uniss.it" class="auth_mail" title="E-mail the corresponding author} ; Luisa Bogliolo^a ; Grazia Galleri^b ; Federica Ariu^a ; Maria Teresa Zedda^a ; Alessandra Pinna^c ; Luca Malfatti^c ; Plinio Innocenzi^c ; Sergio Ledda^a
• 关键词：Nanoceria ; Semen ; Kinematic parameter ; Biocompatibility
• 刊名：Theriogenology
• 出版年：2016
• 出版时间：15 April 2016
• 年：2016
• 卷：85
• 期：7
• 页码：1274-1281.e3
• 全文大小：1403 K

文摘

The aim of the study was to investigate the interaction and the short-term effects of increasing doses of cerium dioxide nanoparticles (CeO₂ NPs) on ram spermatozoa, stored at 4 °C for up to 24 hours, on the main functional and kinematic parameters. Spermatozoa were incubated with 0, 22, 44, and 220 μg/mL of CeO₂ NPs at 4 °C and submitted at 0, 2, and 24 hours to the following analyses: (1) intracellular uptake of CeO₂ NPs by the spermatozoa; (2) kinematic parameters; (3) acrosome and membrane integrity; (4) integrity of DNA; (5) mitochondrial activity; (6) ROS production. The results indicated that the exposure of spermatozoa to increasing doses of nanoceria was well tolerated. No intracellular uptake of NPs by the cells was observed and both kinematic parameters and status of the membranes were not affected by the incubation with NPs (P > 0.05). Moreover, no influence on the redox status of spermatozoa and on the levels of fragmentation of DNA was reported among groups at any time (P > 0.05). The data collected provide new information about the impact of CeO₂ NPs on the male gamete in large animal model and could open future perspectives about their biomedical use in the assisted reproductive techniques.