Cloning and characterization of Rab Escort Protein (REP) from Arabidopsis thaliana
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- 作者：M. Wojtas ; S. Swiezewski ; T.J. Sarnowski ; D. Plochocka ; A. Chelstowska ; T. Tolmachova ; E. Swiezewska
- 关键词：Rab Escort Protein ; Rab GGTase ; GGTase II ; Arabidopsis thaliana
- 刊名：Cell Biology International
- 出版年：2007
- 出版时间：March 2007
- 年：2007
- 卷：31
- 期：3
- 页码：246-251
- 全文大小：629 K
文摘
Intracellular vesicular trafficking is regulated by Rab proteins, small GTPases that require posttranslational geranylgeranylation for biological activity. This covalent modification is catalyzed by Rab geranylgeranyl transferase (RabGGTase) and proceeds only in the presence of accessory Rab Escort Protein (REP). In this communication, we report the cloning and characterization of REP gene of Arabidopsis thaliana. Highest expression of REP mRNA was detected in leaves and flowers in contrast to stems and roots. AtREP is recognized by anti-rat REP1 serum. Interaction of AtREP with the protein substrate is presented, as well as a structural model obtained through homology modeling, based on the known structure of rat REP1.