Differential regulation of neuropeptide Y receptors in the brains of NPY knock-out mice

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• 作者：Trivedi ; Prashant G. ; Yu ; Hong ; Trumbauer ; Myrna ; Chen ; Howard ; Van der Ploeg ; Lex H.T. ; Guan ; Xiao-Ming
• 关键词：NPY Receptor regulation ; Expression ; mRNA ; Receptor binding ; NPY Knockout mouse
• 刊名：Peptides
• 出版年：2001
• 出版时间：March, 2001
• 年：2001
• 卷：22
• 期：3
• 页码：395-403
• 全文大小：1.96 M

文摘

To study the effect of NPY deletion on the regulation of its receptors in the NPY knockout (NPY KO) mice, the expression and binding of NPY receptors were investigated by in situ hybridization and receptor autoradiography using ¹²⁵I-[Leu³¹,Pro³⁴]PYY and ¹²⁵I-PYY_3-36 as radioligands. A 6-fold increase in Y2 receptor mRNA was observed in the CA1 region of the hippocampus in NPY KO mice, but a significant change could not be detected for Y1, Y4, Y5 and y6 receptors. Receptor binding reveals a 60–400 % increase of Y2 receptor binding in multiple brain areas. A similar increase in Y1 receptor binding was seen only in the hypothalamus. These results demonstrate the NPY receptor expression is altered in mice deficient for its natural ligand.