Synthetic glycosylphosphatidylinositol microarray reveals differential antibody levels and fine specificities in children with mild and severe malaria
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- 作者：Marco Tamborrini ; Xinyu Liu ; Joseph Paschal Mugasa ; Yong-Uk Kwon ; Faustin Kamena ; Peter H. Seeberger ; Gerd Pluschke
- 关键词：Malaria ; Antibody responses ; GPI ; Synthetic oligosaccharides ; Microarrays
- 刊名：Bioorganic and Medicinal Chemistry
- 出版年：2010
- 出版时间：1 June 2010
- 年：2010
- 卷：18
- 期：11
- 页码：3747-3752
- 全文大小：392 K
文摘
Glycosylphosphatidylinositol (GPI) glycolipids abound on the cell surface at the merozoite stage of Plasmodium falciparum life cycle are a central toxin in malaria. The contribution of GPI specific humoral immune responses to protection against malaria pathology is not clear, since studies on the correlation between anti-GPI antibody titers and disease severity have yielded contradictory results. Here, we present the application of a carbohydrate microarray based on synthetic PfGPI glycans to assess levels and fine specificities of anti-GPI antibody responses in healthy and malaria diseased individuals. Furthermore, the age dependent development of humoral immune responses against GPI in malaria-exposed children was investigated. Anti-GPI antibodies were only rarely found in children under the age of 18 months. Sera from subjects with severe malaria and healthy children contained antibodies that recognized predominantly synthetic Man3-GPI and Man4-GPIs. In contrast, antibodies in sera of children with mild malaria also showed substantial reactivity with truncated glycans comprising glucosamine–inositol moieties without mannose or with only one or two mannose residues.