Development of a multiplex taqMan real-time PCR assay for typing of Mycoplasma pneumoniae based on type-specific indels identified through whole genome sequencing

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• 作者：Bernard J. Wolff ; Alvaro J. Benitez ; Heta P. Desai ; Shatavia S. Morrison ; Maureen H. Diaz ; Jonas M. Winchell ; ^{jwinchell@cdc.gov}
• 关键词：M. pneumoniae ; P1 typing ; Real-time PCR ; Next generation sequencing
• 刊名：Diagnostic Microbiology and Infectious Disease
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：87
• 期：3
• 页码：203-206
• 全文大小：918 K
• 卷排序：87

文摘

We developed a multiplex real-time PCR assay for detecting and typing M. pneumoniae. Typing was based on the presence of short type-specific indels for P1 typing. This assay was 100% specific compared to existing methods.