A novel extracellular cold-active esterase of Pseudomonas sp. TB11 from glacier No.1: Differential induction, purification and characterisation
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- 作者：Juan Donga ; c ; dongjuanvv@126.com" class="auth_mail" title="E-mail the corresponding author ; Wei Zhaoa ; b ; zhaow@jiangnan.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Mohammed A.A. Gasmallab ; Jingtao Sunc ; Xiao Huaa ; b ; Wenbin Zhanga ; b ; Liang Hanb ; Yuting Fanb ; Yinghui Fengb ; Qiuyun Shenb ; Ruijin Yanga ; b ; yrj@jiangnan.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：EstTB11 ; a cold-adapted esterase from psychrotolerant Pseudomonas sp. TB11 ; pNPA ; p-nitrophenyl acetate ; pNPB ; p-nitrophenyl butanoate ; p ; NPCp-nitrophenyl caprylate ; pNPD ; p-rophenyl decanoate ; pNPL ; p-nitrophenyl laurate ; pNPP ; p-nitrophenyl palmitate ; p ; NPSp-nitrophenyl tearate ; ARTP ; atmospheric and room temperature plasma ; PVA ; polyvinyl alcohol ; pNP ; p-nitrophenol ; SDS-PAGE ; sodium dodecyl sulfate polyacrylamide gel electrophoresis ; MALDI-TOF-MS ; matrix-assisted laser desorption/ionisatio
- 刊名：Journal of Molecular Catalysis B: Enzymatic
- 出版年：2015
- 出版时间：November 2015
- 年：2015
- 卷：121
- 期：Complete
- 页码：53-63
- 全文大小：1971 K
文摘
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Single cream was found as a good inducer for esterase to catalyze short and medium—a novel esterase from a psychrotolerant strain of glacier No.1 was purified and characterized.
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Chain fat.
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An improved zymography method was developed, which is simpler and faster.
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It was proved EstTB11 had potential application in flavor development of dairy products.