Immuno-detection of OCTN1 (SLC22A4) in HeLa cells and characterization of transport function
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- 作者：Lorena Pochini ; Mariafrancesca Scalise ; Cesare Indiveri ; cesare.indiveri@unical.it" class="auth_mail" title="E-mail the corresponding author
- 关键词：TEA ; tetraethylammonium ; PLP ; pyridoxal 5-phosphate ; MTSEA ; 2-aminoethyl methanethiosulfonate ; VAChT ; vesicular acetylcholine transporter ; OCT ; organic cation transporter ; OCTN ; organic cation transporter novel ; ChT ; choline transporter
- 刊名：International Immunopharmacology
- 出版年：2015
- 出版时间：November 2015
- 年：2015
- 卷：29
- 期：1
- 页码：21-26
- 全文大小：613 K
文摘
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OCTN1 was immunodetected in HeLa cells by a specific antirecombinant OCTN1 antibody.
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HeLa OCTN1 mediated acetylcholine and choline uptake and efflux in proteoliposomes.
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HeLa OCTN1 was regulated by internal ATP as the recombinant protein.
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Acetylcholine transport was inhibited by PLP and MTSEA and other OCTN1 inhibitors.
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Acetylcholine uptake and efflux occurred in intact HeLa cells as well.