HaloTag™: Evaluation of a covalent one-step immobilization for biocatalysis

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• 作者：Johannes Dö ; bber ; Martina Pohl ; ^{ma.pohl@fz-juelich.de}
• 关键词：MBP ; maltose binding protein ; PfBAL ; benzaldehyde lyase from Pseudomonas fluorescence ; HPP ; hydroxy-1-phenylpropan-1-one ; ThDP ; Thiamine diphosphate
• 刊名：Journal of Biotechnology
• 出版年：2017
• 出版时间：10 January 2017
• 年：2017
• 卷：241
• 期：Complete
• 页码：170-174
• 全文大小：814 K
• 卷排序：241

文摘

The HaloTag is a useful fusion partner to mediate covalent enzyme immobilization. HaloTag enables purification and immobilization in one step from crude cell extracts. The strategy was successfully tested with benzaldehyde lyase from P. fluorescens.