Biodegradation of oxyethylated fatty alcohols by bacteria Microbacterium strain E19

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• 作者：Dorota Nowicka ; Dobrochna Ginter-Kramarczyk ; Aleksandra Holderna-Odachowska ; Irena Budnik ; Ewa Kaczorek ; Zenon Lukaszewski<sup> ; sup><sup>zenon.lukaszewski@plusnet.plsup><sup> ; sup><sup>Zenon.Lukaszewski@put.poznan.plsup>
• 关键词：Oxyethylated fatty alcohols ; Biodegradation ; Metabolites ; LC&ndash ; MS ; Ethoxylate &lsquo ; fingerprints&rsquo ; Microbacterium sp
• 刊名：Ecotoxicology and Environmental Safety
• 出版年：2013
• 出版时间：1 May, 2013
• 年：2013
• 卷：91
• 期：Complete
• 页码：32-38
• 全文大小：927 K

文摘

The Microbacterium sp. E19 (E19) has been isolated from soil contaminated with crude oil and is a candidate for surfactant enhanced remediation of hydrocarbon polluted soil. Oxyethylated alcohols (OA) are candidates for this process enhancement. The aim of this work was the investigation of biodegradation of a representative oxyethylated fatty alcohol (polydispersal surfactant C<sub>12sub>E<sub>10sub>(C<sub>12sub>E<sub>10sub>)) by E19 under static model conditions with the surfactant as a sole source of organic carbon. LC-MS was used for the identification of metabolites and determination of surfactant and metabolite concentrations. Apart from [M+NH4]<sup>+sup> ethoxylate ¡®fingerprints¡¯, [M+2NH4]<sup>++sup> ¡®fingerprints¡¯ (m/z=22) were used for the identification of particular species. Primary biodegradation of C<sub>12sub>E<sub>10sub> by E19 is almost complete over 30 days of the test (97 percent). The major metabolites during the initial period of the test are homologs of oxyethylated alcohols ¦Ø-carboxylated in the oxyethylene chain and poly(ethylene glycols). 1/3 of the total C<sub>12sub>E<sub>xsub> is metabolized along this pathway. Concentration of these metabolites is stable over the subsequent days of the test. Further biodegradation of C<sub>12sub>E<sub>xsub> causes an enrichment of the residue with C<sub>12sub>E<sub>xsub> homologs having a longer oxyethylene chain. However, intermediates of this process were not identified.