Antioxidant properties, total phenols and pollen analysis of propolis samples from Portugal

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• 作者：Le ; ro Moreira ; Luí ; s G. Dias ; José ; Alberto Pereira ; Leticia Estevinho
• 关键词：Portugal ; Propolis ; Polinic analysis ; Total phenols ; Antioxidant activity
• 刊名：Food and Chemical Toxicology
• 出版年：2008
• 出版时间：November 2008
• 年：2008
• 卷：46
• 期：11
• 页码：3482-3485
• 全文大小：240 K

文摘

Pollen analysis, total phenols content and antioxidant activity were studied for the first time in Portuguese propolis samples from Bornes and Fundão regions. Total phenols content was determined by colorimetric assay and their amount was of 329 mg/g of GAE in Bornes sample and 151 mg/g of GAE in Fundão propolis. The antioxidant capacity of propolis extracts was assessed through the scavenging effects on DPPH (2,2-diphenyl-1-picrylhydrazyl) and reducing power of iron (III)/ ferricyanide complex assays. A concentration-dependent antioxidative capacity was verified in DPPH and reducing power assays. Low values of EC₅₀ on DPPH scavenging assay were obtained for Bornes and Fundão propolis (of 6.22 μg/mL and 52.00 μg/mL, respectively). For reducing power the values were 9.00 μg/mL, for Bornes propolis, and 55.00 μg/mL, for Fundão propolis. The high activity of propolis from Bornes could be related with their different pollen composition. The results obtained indicate that Portuguese propolis is an important source of total phenols showing antioxidant properties that could be beneficial for human health.