Detection of alpha human papillomaviruses in archival formalin-fixed, paraffin-embedded (FFPE) tissue specimens
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- 作者：Bo&scaron ; tjan J. Kocjana ; Lea Ho&scaron ; njaka ; Mario Poljaka ; b ; mario.poljak@mf.uni-lj.si" class="auth_mail" title="E-mail the corresponding author
- 关键词：Human papillomaviruses ; HPV ; Achival tissues specimens ; Formalin fixed ; Paraffin-embedded tissue ; FFPE
- 刊名：Journal of Clinical Virology
- 出版年：2016
- 出版时间：March 2016
- 年：2016
- 卷：76
- 期：supp_S1
- 页码：S88-S97
- 全文大小：152 K
文摘
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FFPE tissues are an invaluable source for diagnostic and research purposes.
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Several methods for HPV DNA/RNA extraction from FFPE tissues are available.
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Commercial FFPE DNA/RNA extraction kits are rarely used in HPV research.
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HPV PCR-protocols based on SPF10 or GP5/6+ primers perform best on FFPE tissues.
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FFPE tissues can be used for next-generation sequencing.