Immunoproteomics of tree of heaven (Ailanthus atltissima) pollen allergens

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• 作者：Fateme Mousavi^a ; Ahmad Majd^a ; ^b ; Youcef Shahali^c ; ^{y.shahali@rvsri.ac.ir} ; ^{youcef.shahali@persiflore.fr} ; Farrokh Ghahremaninejad^a ; Raheleh Shokouhi Shoormasti^d ; Zahra Pourpak^d
• 关键词：AAP ; A. altissima pollen ; I.U.I.S ; International Union of Immunological Societies ; 1D ; one-dimensional gel ; 2D ; two-dimensional gel ; 1-DE ; one-dimensional gel electrophoresis ; 2-DE ; two-Dimensional Gel Electrophoresis ; 2D-PAGE ; two-dimensional- polyacrylamide gel electrophoresis ; PBS ; phosphate buffer saline ; BSA ; bovine serum albumin ; SDS-PAGE ; sodium dodecyl sulfate polyacrylamide gel electrophoresis ; PVDF ; polyvinylidene difluoride ; CBB ; coomassie brilliant blue ; CHAPS ; 3-(3-cholamidopropyl)
• 刊名：Journal of Proteomics
• 出版年：2017
• 出版时间：10 February 2017
• 年：2017
• 卷：154
• 期：Complete
• 页码：94-101
• 全文大小：1137 K
• 卷排序：154

文摘

Tree of heaven: a highly invasive plant species and an emerging cause of pollen allergy worldwide. The first attempt to identify pollen allergens from this plant family (Simaroubaceae). From about 125 2-DE protein spots, 13 were IgE reactive and 3 candidate allergens was identified. Two IgE binding proteins i.e. enolase and pectate lyase are well-known pollen allergens. The third one is calreticulin, already reported as allergen in molds and helminths.