Immobilization of laccase onto poly(glycidylmethacrylate) brush grafted poly(hydroxyethylmethacrylate) films: Enzymatic oxidation of phenolic compounds

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• 作者：Gü ; lay Bayramoğ ; lu ; M. Yakup Arı ; ca
• 关键词：ATRP ; Polymer brushes ; Immobilized enzyme ; Adsorption ; Laccase ; Phenolic compounds
• 刊名：Materials Science and Engineering: C
• 出版年：2009
• 出版时间：1 August 2009
• 年：2009
• 卷：29
• 期：6
• 页码：1990-1997
• 全文大小：1098 K

文摘

Poly(hydroxyethylmethacrylate), p(HEMA) films were prepared via UV-initiated photo-polymerization. After activation of the hydroxyl groups of p(HEMA) by bromination, surface-initiated atom transfer radical polymerization (ATRP) of glycidylmethacrylate was conducted in dioxane/bipyridine mixture with CuBr as catalyst at 65 °C. The epoxy groups of the poly(glycidylmethacrylate) brushes were converted into amino groups with the reaction of ammonia. The modified p(HEMA-g-GMA)-NH₂ films were used as an ion-exchange support for the immobilization of laccase. The influence of pH and initial laccase concentration on the immobilization capacity of the p(HEMA-g-GMA)-NH₂ films has been investigated. The amount of immobilized laccase on the p(HEMA-g-GMA)-NH₂ films was determined as 139 μg/cm² films. The recovered activity of the immobilized laccase on the fibrous polymer grafted films was about 71 % compared to free enzyme. The maximum activity (V_max) and Michealis constant (K_m) of laccase immobilized on the films, were found to be 15.4 U/mg and 23 mM, respectively. Finally, the immobilized laccase was operated in a batch system for enzymatic oxidation of phenol, p-chlorophenol and aniline.