MicroRNA-125b as a new potential biomarker on diagnosis of renal ischemia-reperfusion injury

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• 作者：Aydın Gü ; ç ; lü ; MD^a ; ^{aydinguclu@gmail.com} ; Cengiz Koç ; ak ; MD^b ; Fatma E. Koç ; ak ; MD^c ; Raziye Akç ; ılar ; PhD^d ; Yavuz Dodurga ; PhD^e ; Aydın Akç ; ılar ; DVM^f ; Levent Elmas ; BMSc^e
• 关键词：Renal ischemia&ndash ; reperfusion injury ; Bardoxolone methyl ; CDDO-Me ; MicroRNAs ; miR-223-5p ; miR-21 ; miR-125b
• 刊名：Journal of Surgical Research
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：207
• 期：Complete
• 页码：241-248
• 全文大小：3762 K
• 卷排序：207

文摘

Acute renal failure is commonly seen in the perioperative period. Ischemia–reperfusion (IR) injury plays a major role in acute renal failure and delayed graft function. MicroRNAs (miRs), which are pivotal modulators of cell activities, offer a major opportunity for affective diagnosis and treatment strategies because they are tissue specific and in the center of gene expression modulation. The effect of bardoxolone methyl (BM) on miR-21, miR-223-5p, and miR-125b in renal IR injury was evaluated in this study.MethodsWistar-Albino rats (12-16 wk old, weighing 300-350 g) were used in the study. Rats (n = 6) were randomized into three groups (control, IR, and BM + IR). Tissue levels of miRs were analyzed with reverse transcription polymerase chain reaction.ResultsSignificant reduction of urea and total oxidant status, increase of total antioxidant status, and oxidative stress index were identified in the IR + BM group compared with the IR group. Significant increases of miR-21 (2842.82-fold) and miR-125b (536.8-fold) were identified in the IR group compared with the control group; however, miR-223-5p levels did not show any significant difference. Also, miR-21 and miR-125b were significantly reduced in the IR + BM group compared with the IR group. Reduced histopathologic changes were observed in the IR + BM group. A significant decrease in the number of tunel-positive cells was identified in the IR + BM group compared with the IR group.ConclusionsmiR-125b was significantly increased in IR injury; thus, miR-125b can be a potential novel marker that can be used in diagnosis and treatment of renal IR injury. BM reduces miR-21 and miR-125b in case of IR injury and makes functional and histopathologic repairs.