Imaging flow cytometry and GST pulldown assays provide new insights into channel catfish leukocyte immune-type receptor-mediated phagocytic pathways

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• 作者：Myron A. Zwozdesky¹ ; Chenjie Fei¹ ; Dustin M.E. Lillico ; James L. Stafford ; ^{stafford@ualberta.ca}
• 关键词：Phagocytosis ; Immunoregulatory receptors ; Intracellular signaling ; Actin polymerization machinery ; Imaging flow cytometry ; Innate immunity ; Channel catfish ; Leukocyte immune-type receptors
• 刊名：Developmental & Comparative Immunology
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：67
• 期：Complete
• 页码：126-138
• 全文大小：1376 K
• 卷排序：67

文摘

Distinct regions of the IpLITR 1.1b CYT can independently trigger phagocytosis. Distinct regions of the IpLITR 1.1b CYT can differentially recruit intracellular signaling molecules. Imaging flow cytometry can functionally distinguish the phagocytic activities of different IpLITR-types. New models are provided to explain the atypical mode of IpLITR 1.1b-mediated phagocytosis.