Association of interleukin-(IL)10 haplotypes and serum IL-10 levels in the progression of childhood immune thrombocytopenic purpura

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• 作者：Riccardina Tesse ; Giovanni Carlo Del Vecchio ; Domenico De Mattia ; Maria Sangerardi ; Federica Valente ; Paola Giordano
• 关键词：ITP ; Immune thrombocytopenic purpura ; SNPs ; Single nucleotide polymorhisms ; IL-10 ; Interleukin-10 ; PCR ; Polymerase chain reaction ; RFLP ; Restriction fragment length polymorphism ; Treg ; T-regulatory cells ; MHC ; Major Histocompatibility Complex
• 刊名：Gene
• 出版年：2012
• 出版时间：15 August, 2012
• 年：2012
• 卷：505
• 期：1
• 页码：53-56
• 全文大小：176 K

文摘

Derangement of genetic and immunological factors seems to have a pivotal role in the pathophysiology of immune thrombocytopenic purpura (ITP). We investigated interleukin(IL)-10 genetically determined expression in children with an acute progression of ITP (n = 41) compared to young patients with chronic ITP (n = 44) and healthy controls (n = 60), and attempted to correlate IL-10 production with the course of the disease. We genotyped our study population for three single nucleotide polymorphisms at positions ? 1082 (A/G), ? 819 (C/T) and ? 592 (C/A) in the promoter region of the IL-10 gene. IL-10 levels were measured by enzyme-linked immunoassay.

The IL-10 production in our study population was significantly higher in patients carrying the GCC haplotype than those bearing ACC and ATA haplotypes (6.9 ¡À 1.5 vs 3.6 ¡À 0.8 vs 3.3 ¡À 0.3, p = 0.03). The serum concentration of IL-10 was significantly higher in patients with an acute course of their disease, who mainly carried the GCC haplotype (92 % ), compared to chronic subjects, bearing the non-GCC haplotypes, and controls [17 pg/mL (1.7-18) vs 3.5 pg/mL (0.6-11) vs 3 pg/mL (1-7), p < 0.01)]. Our findings show that patients carrying the GCC-high producer IL-10 haplotype have an acute development of ITP and that IL-10 levels might represent a useful predictive biomarker of the disease course.