Evaluation of a commercial real-time PCR kit for the detection of the Q80K polymorphism in plasma from HCV genotype 1a infected patients
详细信息 查看全文
- 作者：Ilaria Vicentia ; ilariavicenti@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Francesca Falascab ; francesca.falasca@uniroma1.it" class="auth_mail" title="E-mail the corresponding author ; Laura Sticchic ; d ; sticchi@unige.it" class="auth_mail" title="E-mail the corresponding author ; Bianca Bruzzonec ; bianca.bruzzone@hsanmartino.it" class="auth_mail" title="E-mail the corresponding author ; Ombretta Turrizianib ; ombretta.turriziani@uniroma1.it" class="auth_mail" title="E-mail the corresponding author ; Maurizio Zazzia ; maurizio.zazzi@gmail.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Drug resistance assay ; Natural polymorphisms ; HCV ; Q80K ; Simeprevir
- 刊名：Journal of Clinical Virology
- 出版年：2016
- 出版时间：March 2016
- 年：2016
- 卷：76
- 期：Complete
- 页码：20-23
- 全文大小：344 K
文摘
- •
Real time PCR kit (Clonit) detects the Q80K variant in plasma from HCV patients.
- •
In an analysis of 43 samples the assay was 100% specific and 97.1% sensitive.
- •
Failure to amplify Q80K in a minority of samples, however no cases of miscalls.
- •
System can assist simeprevir treatment although performance can be improved.