Label-free fluorescent assay of T4 polynucleotide kinase phosphatase activity based on G-quadruplexe−thioflavin T complex

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• 作者：Hengzhi Zhao^a ; Qiang Liu^b ; Mei Liu^b ; Yan Jin^a ; Baoxin Li^a ; ^{libaoxin@snnu.edu.cn}
• 关键词：T4 polynucleotide kinase phosphatase ; Thioflavin T ; Fluorescence assay ; Label-free
• 刊名：Talanta
• 出版年：2017
• 出版时间：1 April 2017
• 年：2017
• 卷：165
• 期：Complete
• 页码：653-658
• 全文大小：476 K
• 卷排序：165

文摘

A label-free and sensitive fluorescence assay for T4 PNKP activity is developed. Commercially available thioflavin T dye is used as signal probe. The whole assay process can be completed in a single tube.