Micropatterning proteins and cells on polylactic acid and poly(lactide-co-glycolide)

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• 作者：Lin ; Chien-Chi ; Co ; Carlos C. ; Ho ; Chia-Chi
• 关键词：Micropatterning ; Poly(lactide-co-glycolide) (PLGA) ; Poly(lactide acid) (PLA) ; Poly(ethylene glycol) ; Tissue engineering
• 刊名：Biomaterials
• 出版年：2005
• 出版时间：June, 2005
• 年：2005
• 卷：26
• 期：17
• 页码：3655-3662
• 全文大小：435 K

文摘

Techniques for micropatterning proteins and cells on biomaterials are important in tissue engineering applications. Here, we present a method for patterning proteins and cells on poly(lactic acid) (PLA) and poly(lactide-co-glycolide) (PLGA) substrates that are routinely used as scaffolds in engineering tissues. Poly(oligoethyleneglycol methacrylate) (poly-OEGMA) or poly(oligoethyleneglycol methacrylate-co-methacrylic acid) (poly(OEGMA-co-MA)) was microcontact printed onto substrates to create cell resistant areas. Proteins adsorbed onto the unprinted regions whereas the polymer printed regions effectively repel non-specific protein adsorption. NIH 3T3 fibroblasts remain confined within the patterns on the PLGA and PLA films for up to 2 weeks and aligned their actin cytoskeleton along the line patterns. In comparison to unpatterned cells, fibroblasts confined within line-shaped patterns show fewer actin filaments. This method for controlling the spatial morphology and distribution of cells on synthetic biomaterials could have significant applications in tissue engineering.