Three polysaccharides (GRPS) were obtained from Gracilaria rubra by hot water. GRPS-1-1, GRPS-2-1 and GRPS-3-2 were all sulfated polysaccharides. GRPS-2-1 and GRPS-3-2 had little toxicity to L-02 cells. GRPS-3-2 exhibited excellent antioxidant and immunostimulating activities in vitro.