Optimization of enzyme-assisted extraction and characterization of polysaccharides from Hericium erinaceus

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• 作者：Yang Zhu ; Qian Li ; Guanghua Mao ; Ye Zou ; Weiwei Feng ; Daheng Zheng ; Wei Wang ; Lulu Zhou ; Tianxiu Zhang ; Jun Yang ; Liuqing Yang ; Xiangyang Wu
• 关键词：Hericium erinaceus polysaccharides ; Enzyme-assisted extraction ; Response surface methodology ; Optimization ; Characterization
• 刊名：Carbohydrate Polymers
• 出版年：30 January, 2014
• 年：2014
• 卷：101
• 期：Complete
• 页码：606-613
• 全文大小：1599 K

文摘

The enzyme-assisted extraction (EAE) of polysaccharides from the fruits of Hericium erinaceus was studied. In this study, response surface methodology and the Box-Behnken design based on single-factor and orthogonal experiments were applied to optimize the EAE conditions. The optimal extraction conditions were as follows: a pH of 5.71, a temperature of 52.03 掳C and a time of 33.79 min. The optimal extraction conditions resulted in the highest H. erinaceus polysaccharides (HEP) yield, with a value 13.46 卤 0.37%, which represented an increase of 67.72% compared to hot water extraction (HWE). The polysaccharides were characterized by FT-IR, SEM, CD, AFM, and GC. The results showed that HEP was composed of mannose, glucose, xylose, and galactose in a molar ratio of 15.16:5.55:4.21:1. The functional groups of the H. erinaceus polysaccharides extracted by HWE and EAE were fundamentally identical but had apparent conformational changes.