Fractionation of Proteins From Whey With Different Pilot Scale Processes

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• 作者：Outinen ; M. ; Tossavainen ; O. ; Tupasela ; T. ; Koskela ; P. ; Koskinen ; H. ; Rantamä ; ki ; P. ; Syvä ; oja ; E.-L. ; et. al.
• 刊名：Lebensmittel-Wissenschaft und -Technologie
• 出版年：1996
• 出版时间：1996
• 年：1996
• 卷：29
• 期：5-6
• 页码：411-417
• 全文大小：141 K

文摘

Pilot scale production of α-lactalbumin (α-La) and β-lactoglobulin (β-Lg) from sweet and acid casein wheys using two heat precipitation and two chromatographic methods was studied. Using the heat precipitation methods, the soluble β-Lg was recovered easily by ultrafiltration, but the recovery of the low density α-La precipitate was difficult. In addition, with acid casein whey, significant denaturation of α-La occurred. β-Lg was selectively removed from acid casein whey with strongly basic silica and polystyrenic anion exchange resin columns by elution with 0.1 mol/L HCl or 0.33 mol/L NaCl solution. β-Lg eluted with HCl was highly denatured.