Oct4-negative multipotent adult progenitor cells and mesenchymal stem cells as regulators of T-cell alloreactivity in mice

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• 作者：Ariane Luyckx¹ ; ^a ; Lien De Somer¹ ; ^a ; Sandra Jacobs^b ; Omer Rutgeerts^a ; Caroline Lenaerts^a ; Valerie D. Roobrouck^c ; Catherine M. Verfaillie^c ; Mark Waer^a ; Stefaan W. Van Gool^b ; An D. Billiau ; ^a ; ^{an.billiau@med.kuleuven.be}
• 关键词：Multipotent adult progenitor cells ; Mesenchymal stem cells ; Immunomodulation ; T-cell alloreactivity
• 刊名：Immunology Letters
• 出版年：2011
• 出版时间：30 June 2011
• 年：2011
• 卷：137
• 期：1-2
• 页码：78-81
• 全文大小：414 K

文摘

Multipotent adult progenitor cells (MAPC) are clinically being explored as an alternative to mesenchymal stem cells (MSC) for the immunomodulatory control of graft-versus-host disease (GvHD). Here, we performed an explorative study of the immunomodulatory potential of mouse MAPC (mMAPC), in comparison with that of MSC (mMSC) using experimental models of T-cell alloreactivity. Suppressive effects of Oct4-expressing mMAPC have been described previously; here, we studied mMAPC expressing low to no Oct4 (‘mClone-3’), recently shown to be most representative for the human MAPC counterpart. mClone-3 and mMSC exhibited similar immunophenotype and in vitro immunogenic behavior. Allogeneic T-cell ↔ dendritic cell-proliferation assays showed strong dose-dependent T-cell-suppressive effects of both mClone-3 and mMSC. In a popliteal lymph node assay, mClone-3 and mMSC equally suppressed in vivo alloreactive T-cell expansion. We conclude that mouse MAPC and MSC exhibit similar immunosuppressive behavior in in vitro and local in vivo GvHD assays.