Increased expression of the chemokine CCL23 in eosinophilic chronic rhinosinusitis with nasal polyps

详细信息    查看全文

• 作者：Julie A. Poposki MS^a ; Ashraf Uzzaman MD^a ; Deepti R. Nagarkar PhD^a ; Regina T. Chustz BSc^a ; Anju T. Peters MD^a ; Lydia A. Suh BSc^a ; Roderick Carter BSc^a ; James Norton MS^a ; Kathleen E. Harris BSc^a ; Leslie C. Grammer MD^a ; Bruce K. Tan MD^b ; Rakesh K. Chandra MD^b ; David B. Conley MD^b ; Robert C. Kern MD^b ; Robert P. Schleimer PhD^a ; Atsushi Kato PhD^a ; ^{a-kato@northwestern.edu" rel="nofollow}
• 关键词：Chronic rhinosinusitis ; nasal polyps ; CCL23 ; CCR1 ; eosinophils ; aspirin sensitivity
• 刊名：Journal of Allergy and Clinical Immunology
• 出版年：2011
• 出版时间：July 2011
• 年：2011
• 卷：128
• 期：1
• 页码：73-81.e4
• 全文大小：1121 K
• ISSN：S0091674911004763

文摘

ass="h4">Background

Chronic rhinosinusitis (CRS) is a heterogeneous chronic disease characterized by local inflammation of the sinonasal tissues. The pathogenesis of CRS remains controversial, but it has been associated with the accumulation of various immune and inflammatory cells in sinus tissue.

ass="h4">Objectives

The objective of this study was to investigate the expression of the chemokine CCL23, which is known to bind to CCR1 and recruit monocytes, macrophages, and dendritic cells, in patients with CRS.

ass="h4">Methods

We collected nasal tissue from patients with CRS and control subjects. We assayed mRNA for CCL23 by using real-time PCR and measured CCL23 protein by means of ELISA, immunohistochemistry, and immunofluorescence.

ass="h4">Results

CCL23 mRNA levels were significantly increased in nasal polyps (NPs) from patients with CRS with nasal polyps (CRSwNP; P&#xa0;< .05) compared with inferior turbinate and uncinate tissue from patients with CRS or control subjects. CCL23 protein levels were also increased in NPs, although these levels were not statistically significant. Immunohistochemical analysis revealed CCL23 expression in mucosal epithelial cells and inflammatory cells, but accumulation of CCL23⁺ inflammatory cells occurred only in NPs. Immunofluorescence data showed CCL23 colocalization with eosinophil cationic protein–positive eosinophils. The concentration of CCL23 in NPs positively correlated with the concentration of eosinophil cationic protein, suggesting that eosinophils are major CCL23-producing cells in NPs. Finally, we found that CCL23 protein levels were significantly increased in NPs from patients with CRSwNP with aspirin sensitivity.

ass="h4">Conclusion

Overproduction of CCL23 in NPs might contribute to the pathogenesis of eosinophilic CRSwNP through the recruitment of CCR1⁺ inflammatory cells, including monocytes and macrophages, and the amplification of local inflammation.