De novo transcriptome analysis of the red seaweed Gracilaria chilensis and identification of linkers associated with phycobilisomes

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• 作者：Marí ; a Alejandra Vorphal^a ; ¹ ; Cristian Gallardo-Escá ; rate^b ; ¹ ; ^{crisgallardo@udec.cl} ; Valentina Valenzuela-Muñ ; oz^b ; Jorge Dagnino-Leone^a ; José ; Aleikar Vá ; squez^a ; José ; Martí ; nez-Oyanedel^a ; Marta Bunster^a
• 关键词：Transcriptome ; Gracilaria chilensis ; Phycobilisomes ; Rhodophyta
• 刊名：Marine Genomics
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：31
• 期：Complete
• 页码：17-19
• 全文大小：366 K
• 卷排序：31

文摘

This work reports the results of the Illumina RNA-Seq of a wild population of female haploid plants of Gracilaria chilensis (Bird et al., 1986) (Rhodophyta, Gigartinalis). Most transcripts were de novo assembled in 12,331 contigs with an average length of 1756 bp, showing that 96.64% of the sequences were annotated with known proteins. In particular, the identification of linker proteins of phycobilisomes (PBS) is reported. Linker proteins have primary been identified in cyanobacteria but the information available about them in eukaryotic red alga is not complete, and this is the first report in G. chilensis. This resource will also provide the basis for the study of metabolic pathways related to polysaccharide production.