Repopulation of porcine kidney scaffold using porcine primary renal cells

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• 作者：Mehran Abolbashari^a ; ¹ ; Sigrid M. Agcaoili^a ; ^b ; ¹ ; Mi-Kyung Lee^a ; ^c ; In Kap Ko^a ; Tamer Aboushwareb^a ; John D. Jackson^a ; James J. Yoo^a ; ^{jyoo@wakehealth.edu" class="auth_mail" title="E-mail the corresponding author} ; Anthony Atala^a
• 关键词：Engineering whole kidney ; Kidney decellularization ; Primary kidney cells ; Recellularization
• 刊名：Acta Biomaterialia
• 出版年：2016
• 出版时间：1 January 2016
• 年：2016
• 卷：29
• 期：Complete
• 页码：52-61
• 全文大小：1728 K

文摘

The only definitive treatment for end stage renal disease is renal transplantation, however the current shortage of organ donors has resulted in a long list of patients awaiting transplant. Whole organ engineering based on decellularization/recellularization techniques has provided the possibility of creating engineered kidney constructs as an alternative to donor organ transplantation. Previous studies have demonstrated that small units of engineered kidney are able to maintain function in vivo. However, an engineered kidney with sufficient functional capacity to replace normal renal function has not yet been developed. One obstacle in the generation of such an organ is the development of effective cell seeding methods for robust colonization of engineered kidney scaffolds. We have developed cell culture methods that allow primary porcine renal cells to be efficiently expanded while maintaining normal renal phenotype. We have also established an effective cell seeding method for the repopulation of acellular porcine renal scaffolds. Histological and immunohistochemical analyses demonstrate that a majority of the expanded cells are proximal tubular cells, and the seeded cells formed tubule-like structures that express normal renal tubule phenotypic markers. Functional analysis revealed that cells within the kidney construct demonstrated normal renal functions such as re-adsorption of sodium and protein, hydrolase activity, and production of erythropoietin. These structural and functional outcomes suggest that engineered kidney scaffolds may offer an alternative to donor organ transplant.

Statement of Significance

15">Kidney transplantation is the only definitive treatment for end stage renal disease, however the current shortage of organ donors has limited the treatment. Whole organ engineering based on decellularization/recellularization techniques has provided the possibility of creating engineered kidney constructs as an alternative to donor organ transplantation. While previous studies have shown that small units of engineered kidneys are able to maintain function in animal studies, engineering of kidneys with sufficient functional capacity to replace normal renal function is still challenging due to inefficient cell seeding methods. This study aims to establish an effective cell seeding method using pig kidney cells for the repopulation of acellular porcine kidney scaffolds, suggesting that engineered kidneys may offer an alternative to donor organ transplant.