Lowering the overall charge on TMPyP4 improves its selectivity for G-quadruplex DNA

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• 作者：Thomas L. Ruan^a ; ¹ ; ⁴ ; Supriya J. Davis^a ; ¹ ; ³ ; Barrett M. Powell^a ; Cole P. Harbeck^a ; ² ; Jan Habdas^b ; Piotr Habdas^c ; Liliya A. Yatsunyk^a ; ^{lyatsun1@swarthmore.edu}
• 关键词：Guanine quadruplex ; Human telomeric DNA ; TMPyP4 derivatives ; FRET ; Binding constants
• 刊名：Biochimie
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：132
• 期：Complete
• 页码：121-130
• 全文大小：1463 K
• 卷排序：132

文摘

Lowering the charge on porphyrins decreases binding affinity but increases selectivity for GQ. Electrostatic interactions play a major role in binding of cationic porphyrins to GQ DNA. Stabilization temperature determined in FRET strongly correlates with porphyrin's charge. Tricationic 4P3 is superior to TMPyP4 in its selectivity and stabilizing ability toward GQ.