In situ characterization of O-linked glycans of Muc2 in mouse colon

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• 作者：Giuseppa Esterina Liquori ; Maria Mastrodonato ; Donatella Mentino ; Giovanni Scillitani ; Salvatore Desantis ; Piero Portincasa ; Domenico Ferri
• 关键词：AAA ; Aleuria aurantia agglutinin ; AB ; alcian blue ; ConA ; Canavalia ensiformis agglutinin ; DBA ; Dolichos biflorus agglutinin ; GSA-II ; Griffonia simplicifolia agglutinin ; HID ; high-iron diamine ; MAA ; Maackia amurensis agglutinin ; PAS ; periodic acid-Schiff
• 刊名：Acta Histochemica
• 出版年：2012
• 出版时间：November, 2012
• 年：2012
• 卷：114
• 期：7
• 页码：723-732
• 全文大小：2198 K

文摘

The characterization of mucus O-linked glycans in the proximal and distal mouse colon was performed by conventional histochemical methods and by lectin histochemistry in combination with enzymatic treatment (PNGase, ¦Á1,2 fucosidase, sialidase digestion), with and without prior desulfation. We demonstrated the presence of sialo- and sulfomucins in both the proximal and distal colon of the mouse. In the distal colon the sulfomucins were clearly prevalent, although there were always sialomucins with sialyl residues linked ¦Á2,6 to the subterminal galactose. Sialic acid was poorly O-acetylated, especially in the distal colon. The lectin binding pattern indicates a massive presence of fucose ¦Á1,2 linked to galactose in O-glycans and smaller quantities of fucose linked ¦Á1,6 to N-acetylglucosamine in the core of N-linked glycans. Lectin histochemistry also demonstrated the presence of glycosidic residues of N-acetylglucosamine, N-acetylgalactosamine, and galactose in oligosaccharide chains of highly sulfated mucins.