Incidence and Outcomes of Positive Donor Corneoscleral Rim Fungal Cultures after Keratoplasty

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• 作者：Jesse M. Vislisel ; MD¹ ; Kenneth M. Goins ; MD¹ ; ³ ; Michael D. Wagoner ; MD ; PhD¹ ; ² ; Gregory A. Schmidt ; BS ; CEBT³ ; Benjamin T. Aldrich ; PhD¹ ; ³ ; Jessica M. Skeie ; PhD¹ ; ³ ; Cynthia R. Reed ; RN ; PhD³ ; M. Bridget Zimmerman ; PhD⁴ ; Mark A. Greiner ; MD¹ ; ² ; ³ ; ^{mark-greiner@uiowa.edu}
• 关键词：CI ; confidence interval ; DALK ; deep anterior lamellar keratoplasty ; DLEK ; deep lamellar endothelial keratoplasty ; DMEK ; Descemet membrane endothelial keratoplasty ; DSAEK ; Descemet stripping automated endothelial keratoplasty ; DSEK ; Descemet stripping endothelial keratoplasty ; EBAA ; Eye Bank Association of America ; EK ; endothelial keratoplasty ; ILEB ; Iowa Lions Eye Bank ; PK ; penetrating keratoplasty ; SD ; standard deviation
• 刊名：Ophthalmology
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：124
• 期：1
• 页码：36-42
• 全文大小：376 K
• 卷排序：124

文摘

To determine the incidence of positive corneoscleral donor rim fungal cultures after keratoplasty and to report clinical outcomes of grafts with culture-positive donor rims.DesignRetrospective cohort study.ParticipantsConsecutive donor corneas and keratoplasty recipients at a single tertiary referral center over 20 years.MethodsPatient charts were reviewed to determine the incidence of positive donor rim fungal cultures and clinical outcomes of all grafts using contaminated tissue.Main Outcome MeasuresThe primary outcome measures were positive donor rim fungal culture results and the development of postkeratoplasty fungal infection using corresponding corneal tissue. The secondary outcome measure was the impact of postoperative prophylaxis on donor tissue-associated infections.ResultsA total of 3414 keratoplasty cases were included in the statistical analysis. Seventy-one cases (2.1%) were associated with a fungal culture-positive donor rim. Candida species were cultured in 40 cases (56.3%). There was a higher incidence of positive rim cultures over the last 5 years of the analytic period compared with the first 15 years (P = 0.018). Fungal keratitis developed in 4 cases (5.6%), and all patients required further surgical intervention to achieve cure. There were no cases of fungal endophthalmitis. Empiric antimycotic prophylaxis initiated at the time of positive culture result reduced the incidence of keratitis from 15.8% in untreated cases to 1.9% in treated cases (P = 0.056).ConclusionsPositive donor rim fungal cultures are uncommon, but carry an unacceptably high risk of postoperative fungal infection. This risk may be reduced with prophylactic antimycotic therapy when culture-positive donor rims are identified.