We identified brain regions involved in metformin-induced anorexia.
Metformin caused weight loss and anorexia in obese mice fed a high fat diet.
Acute metformin activated neurons in the PVN, AP, and CeA.
Repeated metformin activated neurons independent of weight loss and anorexia.
Repeated metformin administration activated neurons in the VMH, NTS, GCL, and Acb.