Corticotropin-releasing factor-like peptide modifies the AMPA-, NMDA-dependent and GABA_B-ergic properties of synaptic transmissions in vitro

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• 作者：Anatoly A. Mokrushin ^{mok@inbox.ru" class="auth_mail}
• 关键词：MTX ; mystixin-7 ; ACSF ; artificial cerebrospinal fluid ; LOT ; lateral olfactory tract ; FP ; field potential ; EPSP ; excitatory postsynaptic potential ; NMDA ; N-methyl-d-aspartate ; AMPA ; alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic ; IPSPs ; postsynaptic potential ; LTP/LTD ; long-term potentiation/depression ; TBS ; 胃 burst stimulation
• 刊名：Regulatory Peptides
• 出版年：10 January, 2014
• 年：2014
• 卷：188
• 期：Complete
• 页码：1-4
• 全文大小：395 K

文摘

The aim of this study was to investigate the neurotrophic effects of the mystixin-7 mini-peptide (MTX, a synthetic corticotrophin-releasing-factor-like peptide-like peptide) using a slice-based system. The technique on-line monitoring of electrophysiological parameters (excitatory glutamatergic AMPAR-, NMDAR-dependent and inhibitory GABA_B-ergic postsynaptic mechanisms) in the olfactory cortex slices of the rat brain exposed to varied amounts of MTX was used. MTX in a dose-dependent manner inhibited both the AMPAR- and NMDAR-mediated postsynaptic processes. The peptide caused depression of inhibitory GABA_B-ergic processes only at low doses of MTX (10, 25, 50 mg/mL) while at higher doses (100, 250 mg/mL) it enhanced them. These effects of MTX were reversible. AMPA-dependent (but not NMDA-mediated mechanisms) and inhibitory processes were restored after washing.

Triple reperfusion of slices with MTX (100 mg/mL) accelerated the inhibitory processes and induced NMDAR desensitization. MTX evoked the long-term depression on 胃 burst stimulation of the slices. This study did not only lead to the conclusion that the functions of the MTX mini-peptide is not limited to anti-inflammatory effects, but also is included modifications of excitatory glutamatergic AMPAR-, NMDAR-dependent and inhibitory GABA_B-ergic postsynaptic mechanisms.