Expression of HPV16 E5 produces enlarged nuclei and polyploidy through endoreplication

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• 作者：Lulin Hu ; Tamara A. Potapova ; Shibo Li ; Susannah Rankin ; Gary J. Gorbsky ; Peter C. Angeletti ; Brian P. Ceresa
• 关键词：HPV ; Endoreplication ; Nuclear morphology ; E5 ; Live cell imaging
• 刊名：Virology
• 出版年：2010
• 出版时间：30 September 2010
• 年：2010
• 卷：405
• 期：2
• 页码：342-351
• 全文大小：1760 K

文摘

Anogenital cancers and head and neck cancers are causally associated with infection by high-risk human papillomavirus (HPV). The mechanism by which high-risk HPVs contribute to oncogenesis is poorly understood. HPV16 encodes three genes (HPV16 E5, E6, and E7) that can transform cells when expressed independently. HPV16 E6 and E7 have well-described roles causing genomic instability and unregulated cell cycle progression. The role of HPV16 E5 in cell transformation remains to be elucidated. Expression of HPV16 E5 results in enlarged, polyploid nuclei that are dependent on the level and duration of HPV16 E5 expression. Live cell imaging data indicate that these changes do not arise from cell–cell fusion or failed cytokinesis. The increase in nuclear size is a continual process that requires DNA synthesis. We conclude that HPV16 E5 produces polyploid cells by endoreplication. These findings provide insight into how HPV16 E5 can contribute to cell transformation.