dsDNA, ssDNA, G-quadruplex DNA, and nucleosomal DNA electrochemical screening using canthin-6-one alkaloid-modified electrodes
详细信息 查看全文
- 作者：Antonio Dom茅nech-Carb贸 ; Gerardo Cebri谩n-Torrej贸n ; Laura de Miguel ; Vicente Tordera ; Daniel Rodrigues-Furtado ; Suzana Assad-Kahn ; Alain Fournet ; Bruno Figad猫re ; Rafael P. V谩zquez-Manrique ; Erwan Poupon
- 关键词：Voltammetry of microparticles ; DNA screening ; G-quadruplex-DNA ; Canthin-6-one ; Natural products
- 刊名：Electrochimica Acta
- 出版年：1 January, 2014
- 年：2014
- 卷：115
- 期：Complete
- 页码：546-552
- 全文大小：1316 K
文摘
Microparticulate films of a canthin-6-one alkaloid (L), a natural 尾-carboline alkaloid presenting a characteristic naphtyridone motif, on glassy carbon electrodes yield different, separate voltammetric signals for dsDNA, ssDNA, G-quadruplex DNA, different degrees of DNA methylation and the biomimetic nucleosomal DNA with detection limit of 10鈭? M. This multiple-signal electrochemical behavior is in contrast with conventional use of DNA intercalators, only discriminating between different DNA forms by variations in the intensity of a unique signal. Complementary photochemical and scanning electrochemical microscopy (SECM) data suggest that the differences in the voltammetric response of the different DNA forms derive from the intercalating properties of the canthin-6-one.