Screening, selection and characterization of phytic acid degrading lactic acid bacteria from chicken intestine
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- 作者：Ponnala Raghavendra ; Prakash M. Halami
- 关键词：Lactic acid bacteria ; Phytic acid ; Phytase ; Acid phosphatase ; Pediococcus pentosaceus
- 刊名：International Journal of Food Microbiology
- 出版年：2009
- 出版时间：31 July 2009
- 年：2009
- 卷：133
- 期：1-2
- 页码：129-134
- 全文大小：232 K
文摘
This study was undertaken to screen and select potent phytate degrading lactic acid bacteria and to evaluate their additional characteristic features. Forty lactic acid bacterial strains were isolated from different sources and screened for their ability to degrade myo-inositol hexaphosphate or IP6 by cobalt chloride staining (plate assay) method, using calcium or sodium salt of phytic acid as substrate. All the forty isolates were able to degrade calcium phytate. However, only two Pediococcus pentosaceus strains (CFR R38 and CFR R35) were found to degrade sodium phytate. These strains showed phytase activity of 213 and 89 U at 50 °C, respectively and poor acid phosphatase activity. These strains were further evaluated for additional characteristic features. At pH 2, P. pentosaceus strains CFR R38 and CFR R35 showed 50.7 and 48.5 percentage survivability after 2 h of incubation respectively and they could also withstand 0.3 % ox-bile. These cultures exhibited 54.6 and 44.8 % of hydrophobicity to xylene, antibacterial activity against food borne pathogens and possessed β-galactosidase activity. The resistance pattern to several antibiotics was also analyzed. The present study indicates that these strains, having phytate degrading ability and other characteristic features can be exploited as starter cultures in fermented foods to improve the mineral bioavailability.