Bioactive albumin functionalized polylactic acid membranes for improved biocompatibility

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• 作者：Gibson S. Nyanhongo ; Rosario D¨ªaz Rodr¨ªguez ; Endry Nugroho Prasetyo ; Cristina Caparr¨®s ; Clarisse Ribeiro ; Vitor Sencadas ; Senentxu Lanceros-Mendez ; Enrique Herrero Acero ; Georg M. Guebitz
• 关键词：Bioactive ; Human serum ; Polylactic acid membranes ; Functionalization ; Cell viability ; Cytocompatibility
• 刊名：Reactive and Functional Polymers
• 出版年：2013
• 出版时间：October, 2013
• 年：2013
• 卷：73
• 期：10
• 页码：1399-1404
• 全文大小：1075 K

文摘

Biocompatibility is a major challenge for successful application of many biomaterials. In this study the ability to coat chemically and enzymatically activated poly(L-lactic acid) (PLA) membranes with heat denatured human serum albumin to improve biocompatibility was investigated. PLA membranes hydrolyzed with NaOH or cutinase and then treated with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide, hydrochloride (EDAC) as a heterobifunctional cross-linker promoted the coupling COOH groups on PLA membranes and NH₂ groups of heat denatured human serum albumin. This resulted in increased hydrophilicity (lowest water contact angles of 43¡ã and 35¡ã) and highest antioxidant activity (quenching of 79 ¦ÌM and 115 ¦ÌM tetramethylazobisquinone (TMAMQ) for NaOH and cutinase pretreated membranes, respectively). FTIR analysis of modified PLA membranes showed new peaks attributed to human serum albumin (amide bond, NH₂ and side chain stretching) appearing within 3600-3000 cm^?1 and 1700-1500 cm^?1 (). MTT studies also showed that osteoblasts-like and MC-3T3-E1 cells viability increased 2.4 times as compared to untreated PLA membranes. The study therefore shows that this strategy of modifying the surfaces of PLA polymers could significantly improve biocompatibility.