Manipulation of HIV-1 gp120-specific immune responses elicited via gene gun-based DNA immunization
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- 作者：Prayaga ; Sudhirdas K. ; Ford ; Maura J. ; Haynes ; Joel R.
- 关键词：DNA immunization ; gene gun ; HIV-1 gp120
- 刊名：Vaccine
- 出版年：1997
- 出版时间：August 9, 1997
- 年：1997
- 卷：15
- 期：12-13
- 页码：1349-1352
- 全文大小：529 K
文摘
Gene gun-based DNA immunization using vectors encoding HIV-1 gp120 or influenza virus nucleoprotein result in Th2-like immune responses following successive immunizations. The codelivery of vectors encoding IL-2, IL-7, or IL-12 blocked this effect by markedly enhancing gp120-specific interferon gamma production, and suppressing IL-4 and IgG1 responses. An unbiased augmentation of all immune responses was observed by increasing the resting period between immunizations. In this case, IFN-γ production following in vitro stimulation increased by over 1000-fold, while IL-4, IgG1, and IgG2a responses were elevated as well. Interestingly, cytokine gene codelivery, in the context of the longer resting period, provided no additional stimulation of Th1-like responses such as IFN-γ and IgG2a production, although there was still some suppression of IL-4 production. These data demonstrate that the quality and magnitude of responses elicited following epidermal administration of DNA vaccines can be manipulated by multiple means.