Renaissance of protein crystallization and precipitation in biopharmaceuticals purification
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- 作者：Raquel dos Santos ; Ana Luí ; sa Carvalho ; almc@fct.unl.pt ; A. Cecí ; lia A. Roque ; cecilia.roque@fct.unl.pt
- 关键词：Protein purification ; Downstream process ; Biopharmaceutical ; Anything but chromatography ; Crystallization ; Precipitation
- 刊名：Biotechnology Advances
- 出版年：2017
- 出版时间：January-February 2017
- 年：2017
- 卷：35
- 期：1
- 页码：41-50
- 全文大小：828 K
- 卷排序：35
文摘
The current chromatographic approaches used in protein purification are not keeping pace with the increasing biopharmaceutical market demand. With the upstream improvements, the bottleneck shifted towards the downstream process. New approaches rely in Anything But Chromatography methodologies and revisiting former techniques with a bioprocess perspective. Protein crystallization and precipitation methods are already implemented in the downstream process of diverse therapeutic biological macromolecules, overcoming the current chromatographic bottlenecks. Promising work is being developed in order to implement crystallization and precipitation in the purification pipeline of high value therapeutic molecules. This review focuses in the role of these two methodologies in current industrial purification processes, and highlights their potential implementation in the purification pipeline of high value therapeutic molecules, overcoming chromatographic holdups.