Prevalidation of the ex-vivo model PCLS for prediction of respiratory toxicity
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- 作者：A. Hessa ; 1 ; L. Wang-Lauensteinb ; c ; 1 ; A. Braunb ; c ; S.N. Kollea ; R. Landsiedela ; M. Liebsche ; L. Ma-Hocka ; R. Pirowe ; X. Schneiderd ; M. Steinfathe ; S. Vogela ; C. Martind ; 1 ; chmartin@ukaachen.de" class="auth_mail" title="E-mail the corresponding author ; K. Sewaldb ; c ; 1 ; katherina.sewald@item.fraunhofer.de" class="auth_mail" title="E-mail the corresponding author
- 关键词：Acute inhalation toxicity ; Respiratory toxicity ; Prevalidation ; Reproducibility ; Industrial chemicals ; Two-group classification model ; Precision-cut lung slices ; Transferability ; Submersed culture conditions
- 刊名：Toxicology in Vitro
- 出版年：2016
- 出版时间：April 2016
- 年：2016
- 卷：32
- 期：Complete
- 页码：347-361
- 全文大小：1413 K
文摘
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Prevalidation of PCLS was successfully conducted in three laboratories using 20 chemicals.
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Intra- and inter-laboratory variabilities showed best consistency and low variability for WST-1 and BCA assays.
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Two prediction models were developed for translation of results from in-vitro to in-vivo.
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The results can potentially be used to predict starting concentrations of acute inhalation toxicity studies.
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Three-dimensional tissue models of the lung provide best correlation to in-vivo data.