Atorvastatin prevents hippocampal cell death, neuroinflammation and oxidative stress following amyloid-β_1–40 administration in mice: Evidence for dissociation between cognitive deficits and neuronal damage

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• 作者：Tetsadê ; C.B. Piermartiri ; Clá ; udia P. Figueiredo ; Daniel Rial ; Filipe S. Duarte ; Sarah C. Bezerra ; Gianni Mancini ; Andreza F. de Bem ; Rui D.S. Prediger ; Carla I. Tasca
• 关键词：Atorvastatin ; Amyloid-β ; peptide ; Neurotoxicity ; Neuroinflammation ; Oxidative stress ; Glutamate ; Spatial learning and memory ; Neuroprotection
• 刊名：Experimental Neurology
• 出版年：2010
• 出版时间：December 2010
• 年：2010
• 卷：226
• 期：2
• 页码：274-284
• 全文大小：1828 K

文摘

The accumulation of amyloid-beta (Aβ) peptides in the brain of human and rodents has been associated with the activation of glial cells, neuroinflammatory and oxidative responses, and cognitive deficits. These oxidative changes leave glutamate transporters more vulnerable and may result in reduction of their functions, resulting in excitotoxic damage. Herein, we evaluated the effects of atorvastatin, a HMG-CoA reductase inhibitor, in molecular and behavioral alterations induced by a single intracerebroventricular injection of aggregated Aβ_1–40 (400 pmol) in mice. An increased glial fibrillar acidic protein (GFAP) expression and cyclooxygenase-2 (COX-2) levels, as well as increased lipid peroxidation and impairment in the glutathione antioxidant system and cell degeneration was found in the hippocampus of Aβ_1–40-treated mice. Aβ_1–40 also induced a marked decrease in glutamatergic transporters (GLAST and GLT-1) expression and in l-[³H] glutamate uptake in mice hippocampus, in addition to spatial learning and memory deficits. Atorvastatin (10 mg/kg/day v.o.) was administered after Aβ_1–40 injection and through seven consecutive days. Atorvastatin treatment was neuroprotective against cell degeneration induced by Aβ_1–40, reducing inflammatory and oxidative responses and increasing the expression of glutamatergic transporters. On the other hand, atorvastatin did not reverse the cognitive impairments and failed to alter the hippocampal glutamate uptake in Aβ_1–40-treated mice. These results reinforce and extend the notion of the potential neuroprotective action of atorvastatin against the neuronal toxicity induced by Aβ_1–40. In addition, the present findings suggest that the spatial learning and memory deficits induced by Aβ peptides in rodents may not be entirely related to neuronal damage.