Proteolytic activity in Encephalitozoon cuniculi sporogonial stages: Predominance of metallopeptidases including an aminopeptidase-P-like enzyme

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• 作者：Patrick Chavant ; Vanessa Taupin ; Hicham El Alaoui ; Ivan Wawrzyniak ; Christophe Chambon ; Gé ; rard Prensier ; Guy Mé ; té ; nier and Christian P. Vivarè ; s
• 关键词：Microsporidia ; Encephalitozoon cuniculi ; Proteolytic activity ; Aminopeptidase P ; Leucine aminopeptidase
• 刊名：International Journal for Parasitology
• 出版年：2005
• 出版时间：2005
• 年：2005
• 卷：35
• 期：13
• 页码：1425-1433
• 全文大小：449 K

文摘

A fraction enriched in spore precursor cells (sporoblasts) of the microsporidian Encephalitozoon cuniculi, an intracellular parasite of mammals, was obtained by Percoll gradient centrifugation. Soluble extracts of these cells exhibited proteolytic activity towards azocasein, with an alkaline optimum pH range (9–10). Prevalence of some metallopeptidases was supported by the stimulating effect of Ca²⁺, Mg²⁺, Mn²⁺ and Zn²⁺ ions, and inhibition by two chelating agents (EDTA and 1,10-phenanthroline), a thiol reductant (dithiothreitol) and two aminopeptidase inhibitors (bestatin and apstatin). Zymographic analysis revealed four caseinolytic bands at about 76, 70, 55 and 50 kDa. Mass spectrometry of tryptic peptides from one-dimensional gel slices identified a cytosol (leucine) aminopeptidase homologue (M17 family) in 50-kDa band and an enzyme similar to aminopeptidase P (AP-P) of cytosolic type (M24B subfamily) in 70-kDa band. Multiple sequence alignments showed conservation of critical residues for catalysis and metal binding. A long insertion in a common position was found in AP-P sequences from E. cuniculi and Nosema locustae, an insect-infecting microsporidian. The expression of cytosolic AP-P in sporogonial stages of microsporidia may suggest a key role in the attack of proline-containing peptides as a prerequisite to long-duration biosynthesis of structural proteins destined to the sporal polar tube.