The role of OsPRA1 in vacuolar trafficking by OsRab GTPases in plant system

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• 作者：Hee Sun Rho ; Jae Bok Heo ; Woo Young Bang ; Sung Min Hwang ; Min Yeop Nahm ; Hyun Jin Kwon ; Sam Woong Kim ; Byung Hyun Lee ; Jeong Dong Bahk
• 关键词：Rab effector ; OsPRA1 ; Prevacuolar compartment (PVC) ; Vacuolar trafficking
• 刊名：Plant Science
• 出版年：2009
• 出版时间：November 2009
• 年：2009
• 卷：177
• 期：5
• 页码：411-417
• 全文大小：1166 K

文摘

PRA1 has been reported as a prenylated Rab acceptor containing GDF activity in human, rat and yeast. Its existence was also proved in plants by sequence analysis, anticipating its important role as a Rab effector, but defined roles of the plant PRA1 homologs remain to be obscure. Here, to get an insight for their role, we performed yeast two-hybrid screen using the OsRab7 as bait and first isolated the OsPRA1, a putative prenylated Rab acceptor, interacting with this protein. Detailed interaction analysis showed that OsPRA1 interacted not only with GDP-bound OsRab7, but also with several other Rab GTPases involved in vacuolar trafficking, in a prenylation-dependent manner. In addition, GFP-fusion analysis demonstrated that OsPRA1 localized to the prevacuolar compartment, and RNA gel blot analysis revealed its significant expression in rice green-aerial tissues such as shoots and mature stems. These results suggest that OsPRA1 may function as a Rab effector for vacuolar trafficking in the plant system.