Determining the cause of recurrent Clostridium difficile infection using whole genome sequencing

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• 作者：James Heng Chiak Sim^a ; Cynthia Truong^a ; Samuel S. Minot^b ; Nick Greenfield^b ; Indre Budvytiene^c ; Akshar Lohith^d ; Victoria Anikst^a ; Nader Pourmand^d ; Niaz Banaei^a ; ^c ; ^e ; ^{nbanaei@stanford.edu}
• 关键词：Clostridium difficile ; Relapse ; Reinfection
• 刊名：Diagnostic Microbiology and Infectious Disease
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：87
• 期：1
• 页码：11-16
• 全文大小：368 K
• 卷排序：87

文摘

Understanding the contribution of relapse and reinfection to recurrent Clostridium difficile infection (CDI) has implications for therapy and infection prevention, respectively. We used whole genome sequencing to determine the relation of C. difficile strains isolated from patients with recurrent CDI at an academic medical center in the United States. Thirty-five toxigenic C. difficile isolates from 16 patients with 19 recurrent CDI episodes with median time of 53.5 days (range, 13–362) between episodes were whole genome sequenced on the Illumina MiSeq platform. In 84% (16) of recurrences, the cause of recurrence was relapse with prior strain of C. difficile. In 16% (3) of recurrent episodes, reinfection with a new strain of C. difficile was the cause. In conclusion, the majority of CDI recurrences at our institution were due to infection with the same strain rather than infection with a new strain.