Cytotoxic effect against 3T3 fibroblasts cells of saffron floral bio-residues extracts

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• 作者：J茅ssica Serrano-D铆az ; Carmen Estevan ; M. 脕ngel Sogorb ; Manuel Carmona ; Gonzalo L. Alonso ; Eugenio Vilanova
• 关键词：TFA ; trifluoroacetic acid ; HCl ; hydrochloric acid ; TPC ; total phenolic content ; TAC ; total anthocyanin content ; K3-S ; kaempferol 3-sophoroside ; Q3-S ; quercetin 3-sophoroside ; K3-S-7G ; kaempferol 3-sophoroside-7-glucoside ; I3 ; 4&prime ; -diG ; isorhamnetin 3 ; 4&prime ; -diglucoside ; K3-R ; kaempferol 3-rutinoside ; K3-G ; kaempferol 3-glucoside ; K ; kaempferol ; D3 ; 5-diG ; delphinidin 3 ; 5-diglucoside ; P3 ; 5-diG ; petunidin 3 ; 5-diglucoside ; D3-G ; delphinidin 3-glucoside ; M3 ; 5-diG ; malvidin 3 ; 5-diglucoside ; MTT
• 刊名：Food Chemistry
• 出版年：15 March, 2014
• 年：2014
• 卷：147
• 期：Complete
• 页码：55-59
• 全文大小：298 K

文摘

For every kilogram of saffron spice produced, about 63 kg of floral bio-residues (FB) (tepals, stamens and styles) are thrown away. Extracts of these bio-residues in water (W1), water:HCl (100:1, v/v) (W2), ethanol (E3), ethanol:HCl (100:1, v/v) (E4), dichloromethane (D5) and hexane (H6) were prepared. Their composition in flavonols and anthocyanins, and their effect on cell viability were determined. W1 was the richest in kaempferol 3-sophoroside (30.34 mg/g dry FB) and delphinidin 3,5-diglucoside (15.98 mg/g dry FB). The highest tested concentration (900 渭g/ml) of W1, W2, E4, D5 and H6 did not significantly decrease the cell viability. Only E3 at that concentration caused a significant decrease of 38% in the cell viability. Therefore, all extracts studied are not cytotoxic at concentrations lower than 900 渭g/ml, and W1 is proposed as the optimal for food applications due to its greater contribution of phenolic compounds.