Effective synthesis of theaflavin-3,3′-digallate with epigallocatechin-3-O-gallate and epicatechin gallate as substrates by using immobilized pear polyphenol oxidase

详细信息    查看全文

• 作者：Shicheng Lei^a ; ^b ; Minhao Xie^a ; Bing Hu^a ; Li Zhou^a ; Yi Sun^a ; Muhammad Saeeduddin^a ; Hongcheng Zhang^c ; Xiaoxiong Zeng^a ; ^{zengxx@njau.edu.cn}
• 关键词：Polyphenol oxidase ; Huangguan pear ; Fe3O4/chitosan nanoparticles ; Immobilization ; Theaflavin-3 ; 3&prime ; -digallate ; Synthesis
• 刊名：International Journal of Biological Macromolecules
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：94
• 期：part_PA
• 页码：709-718
• 全文大小：1407 K
• 卷排序：94

文摘

Polyphenol oxidase from pear was purified 24.36-fold in specific activity. Purified pear PPO was successfully immobilized onto Fe3O4/chitosan nanoparticles. Theaflavin-3,3′-digallate was synthesized with EGCG and ECG by using immobilized PPO. The maximum yield of TF3 was 42.23%. The immobilized PPO exhibited excellent storage stability and reusing.