Identification and characterization of microRNAs in turmeric (Curcuma longa L.) responsive to infection with the pathogenic fungus Pythium aphanidermatum
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- 作者：Subodh Kumar Chand1 ; Satyabrata Nanda1 ; Ellojita Rout ; Jatindranath Mohanty ; Rukmini Mishra ; Raj Kumar Joshi ; rajjoshi@soauniversity.ac.in" class="auth_mail" title="E-mail the corresponding author ; rajkumar.joshi@yahoo.co.in" class="auth_mail" title="E-mail the corresponding author
- 关键词：Pythium soft rot ; Pythium aphanidermatum ; Turmeric ; miRNAs ; Differential expression ; RLM-5&prime ; RACE
- 刊名：Physiological and Molecular Plant Pathology
- 出版年：2016
- 出版时间：January 2016
- 年：2016
- 卷：93
- 期：Complete
- 页码：119-128
- 全文大小：2234 K
文摘
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28 miRNAs responsive to Pythium aphanidermatum were identified from infected turmeric rhizomes.
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5">Stem-loop reverse transcription PCR validated the expression of representative miRNAs from different families.
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RLM-5′-RACE analyses identified and validated distinct miRNA targets.
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5">qPCR revealed simultaneous reciprocal changes in the expression pattern of miRNAs and the target transcripts.
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Turmeric miR164, miR393, miR482 and miRx2 were pried by P. aphanidermatum to facilitate pathogenesis.